For the Female Hormone Health Check we analyse:

Estradiol, Progesterone, Testosterone, DHEA and Cortisol

What are these hormones for?

Estradiol and Progesterone

Estrogens are the most important of the female sex hormones. Estrogen is actually made up of a group of hormones, but Estradiol is biologically the most active during the fertile years of women.

It is responsible for:

-The development of the female body, from girl to woman

-The monthly regeneration and protection of the endometrium (womb)

-Breast growth

-The elasticity of the blood vessels

-Radiant skin and healthy hair

-Moist eyes and mucous membranes

-Clear thinking processes

-Stabilisation of moods

Progesterone is produced by the ovaries shortly after ovulation in order to prepare the uterus for a possible pregnancy and to protect the mucous lining of the uterus. Together with Estrogen, the most important of the female sex hormones, progesterone regulates the menstrual cycle.

The sensitive balance between estrogen and progesterone

Estrogen values tend to fluctuate throughout a woman’s lifetime, not just during the menstrual cycle. The strength of these fluctuations is different for each individual and the body reacts to them with different symptoms. It is not just a deficiency in Estrogen that can cause physical complaints: an excess, or Estrogen dominance, can cause a variety of unpleasant symptoms, such as water retention, nausea, depressive moods, tiredness, listlessness, lack of concentration, anxiety, breast sensitivity, cysts and fibroids, painful menstruation, and weight gain.

Although it may sound contradictory, an Estrogen dominance can occur at the same time as an Estrogen deficiency due to the fine, coordinated balance between Estrogen and progesterone. The Estrogen levels define how much progesterone is needed. An Estrogen dominance can occur if the body is not producing enough progesterone for the level of Estrogen. This means it can also occur if the body is not producing enough Estrogen (Estrogen deficiency) if the body is also not producing the relative amount of progesterone.

Therefore, in the case of an Estrogen deficiency it is important to not only determine the absolute values of the individual hormones, but also the ratio of Estrogen to progesterone. 

DHEA and Cortisol

Cortisol and DHEA are both produced in the adrenal glands. Cortisol is the most important stress hormone besides adrenaline: Stress hormones help the body to react to particular strain. They release energy reserves in order to prepare the body for “escape” or “attack”. During hard, physical work, competitive sports, psychological, physical and mental stress situations, or during a severe illness, the distribution of cortisol is also stimulated. 

Cortisol and DHEA play an important role in the different metabolic processes in the body. In immune processes, cortisol helps with its anti-inflammatory and anti-allergic effect, and has an important function in maintaining the health of the body. 

DHEA is a precursor of female and male sex hormones. The production of DHEA declines with advancing age from the age of 30 and its deficiency can cause various diseases.

It is responsible for:

-Burning of body fat

-Prevention of osteoporosis

-Minimizing cancer and heart diseases

-Strengthening the immune system

-Slowing down the aging process 

-Positively influence memory performance 

However: Both too low and too high levels can cause unpleasant symptoms.

It is well known that chronic stress is unhealthy – and stress is, in turn, the most important impulse that causes the increase of the distribution of cortisol. If the cortisol levels are too high, symptoms can include immunodeficiency, depression, physical exhaustion, metabolic disorders, diabetes, obesity and sleeping disorders. Excessively high cortisol levels can indeed cause unpleasant symptoms.

Just the same, excessively low cortisol levels can interfere with general well-being: Cortisol is vital. Apart from the provision of energy reserves, it also plays a role in the cardiovascular health,gastrointestinal health, the immune system and brain functions. As does DHEA. Low DHEA levels are susprected to increase the risk of Alzheimer’s disease, cancer, osteoporosis, depression, heart disease and obesity. High DHEA levels can cause in women a deeper voice and an increased hair growth all over the body.

Testosterone

Testosterone is a sex hormone that is mainly associated with men – after all, it is responsible for the characteristics that distinguish the appearance of men from women, such as beard growth: it ensures the development of the male sex organ and is responsible for sexual potency and fertility. In addition, it strengthens the heart, brain and bones, supports muscle growth and fat decomposition, counteracts diabetes and high blood pressure, and helps to lower cholesterol levels. 

However, testosterone is also important for women, even though they produce 20 to 30 times less testosterone than men. Testosterone has a mood lifting effect, ensures for more assertiveness and enhances the desire for sex in both women and men. It also enhances sexual sensitivity, making it easier for women to reach orgasm.

The body can only profit from all of these positive properties by having a healthy testosterone level; a deficiency can cause the complaints listed above and an excess can, amongst other things, lead to excessive aggressiveness, acne, oily skin and unwanted body hair (face and body) in women.

Testosterone deficiency can affect women as well as men.

Some of the symptoms connected with a testosterone deficiency:

-Decreased libido, potency problems (♂), orgasm difficulties (♀)

-Decrease in muscles / few distinctive muscles

-Fat deposits in the stomach and breast areas

-Cellulite, varicose veins

-Slack arms and face, wrinkle development, dry skin

-Decreased hair growth (e.g. beard (♂))

-Low stamina, consistent tiredness

-Low assertiveness, uncertainty

-Decreased stress resistance, nervousness

-Anxiety, depressive moods

-Forgetfulness

For the Comprehensive Gut Biome & Health Test we analyse:

Escherichia coli

Escherichia coli (E. coli) is a minority member of the gut microbiome, but a very important one. E. coli bacteria normally live in the intestines of healthy people and stimulates the intestinal immune system. It aids digestion and protects us from other harmful microbes. E. coli ferments to produce folic acid, vitamin K2 (this protects against osteoporosis) and Co-enzyme Q10 (essential for mitochondrial function). If there are low counts of E-coli, one can expect problems like osteoporosis and bone problems, mitochondrial function, low mood and poor gut motility. Increased E. coli indicates putrefaction in the intestine and may burden the body with metabolic toxins. Potential causes may be reduced immunity of the intestinal mucosa, low counts of bifidobacteria or excess supply of protein.

Proteus

Proteus species are most commonly found  as part of normal human intestinal flora, along with Escherichia coli and Klebsiella species. Proteus is also found in multiple environmental habitats, including long-term care facilities and hospitals. As a particularly active proteolytic germ, proteus can burden the body considerably through its metabolic toxins. 

Citerobacter

Citrobacter is a rod belonging to the Enterobacteriaceae family. It is considered an opportunistic pathogen and therefore can be found in the gut as part of the normal flora. Citrobacter are also commonly found in water, soil and food and may be spread by person-to person contact.

Klebsiella

Klebsiella is a bacterium, which belongs to the Enterobacteriaceae family. Klebsiella can be found in the gastrointestinal tract of humans. Klebsiella overgrowth is commonly asymtomatic. Some strains of Klebsiella may cause diarrhea and some are enterotoxigenic. A low-starch diet may be helpful if high levels of Klebsiella are present.

Other enterobacteriaceae 

Increased enterobacteriaceae indicate disturbances of the intestinal flora, malnutrition or digestive insufficiencies. A larger amount of these bacteria does not belong in the normal intestinal flora. Their multiplication often results from past antibioses. 

Enterococci

Decreased Enterococci in the stool indicates disturbances in balance of the intestinal flora. Enterococci produce short-chain fatty acids during the carbohydrate utilisation, which contributes to the acidification and stabilisation of the intestinal environment. As an important contribution to the intestinal milieu, they produce bacteriostatic substances. 

Pseudomonas

Pseudomonas can be found in water and soil as well as fruits ans vegetables. A common source of infection is bottled water, but increased Pseudonomas may also be due to an earlier antibiotic therapy.

Bacteroides 

Bacteroides is the most abundant bacteria in the microflora, which allows us to digest soluble fibre and make short chain fatty acids. Decreased bacteroides indicate a lowered resistence to pathogenic species (such as salmonella, shigella and clostridia). 

Bifidobacteria

Bifidobacteria make up a significant portion of the human gut flora. Along with Lactobacillia and Enterococci, Bifidobacteria control potentially pathogenic organisms and stimulate the intestinal immune system (GALT). Bifidobacteria metabolize carbohydrates only. By doing so, they produce short chain fatty acids, which adidify the intestine and couteract pathogenic organisms. Decreased Bifidobacteria indicate deficiencies in colonisation resistance, putrefaction in the intestine and can promote constipation.

Lactobacilli

Lactobacilli is a lactic acid forming bacteria, which produces large amounts of short chain fatty acids (SCFA). SCFAs lower the intestinal pH and thereby make the evironment alkaline and unsuitable for microbial pathogens (e.g. yeast).
In addition, Lactobacilli secrete antifungal and antimicrobial agents. Decreased Lactobacilli indicate disturbances of the intestinal flora.

Clostridia

Clostridia are prevalent flora in a healthy intestine. As clostirida produce gases it may cause flatulence. Increased Clostridia indicates putrefaction in the intestine and may burden the body with metabolic toxins. Increased clostridia are often found in older people due to changes in their nutrition. Clostridium difficile and Clostridium perfringens are one cause of antibiotic-associated diarrhea.

pH-Value

Fecal pH is influenced by numerous factors, but is largely dependet on the fermentation of fiber by the beneficial flora of the gut. With a balanced diet the fecal pH should lie between 6,2 and 6,8. Acid fecal pH indicates disorders of intestinal ecology. An alkaline fecal pH indicated an imbalanced intestine flora. It is possible that proteolytic putrefactive germs are predominant, which raise the fecal pH through alkaline metabolites (e.g. due to a high-protein diet).

Candida albicans

The genus Candida is comprised of approximately 200 different species. Candida albicans is the most common strain of them. It is a normal part of the gut flora and most people have some level of Candida albicans in their intestines. But a combination of factors can lead to an overgrowth, which then leads to several undesirable symptoms including fatigue, weight gain, joint pain, and gas. 

Candida spec.

Increased candida species indicates deficiancies in colonisation resitance, disturbances of the intestinal flora and or a defect mucosa. Candida species may burden the body with toxic metabolites. Some patients respont to even low rates of yeast overgrowth.

Geotrichum candidum

Geotrichum candidum belongs to the Endomyceteaceae family. This organism can be found in soil, dairy products and in human skin and mucosae. Symptoms of Geotrichum infection have been associated with diarrhea and enteritis. 

Yeast

An infection with yeasts can have many reasons. Possible causes are things like corticosteroid therapy, stress, diabetes, malnutrition or birth control pills. All of them weaken the immune system and a weakened immune system cannot control yeast or help you get rid of it. Antibiotics are also a common cause of yeast infection because they destroy the good bacteria that keep it under control.

Helicobacter pylori

The detection of Helicobacter pylori antigen in stool indicates an infection with this germ. Helicobacter pylori is the most common chronic bacterial pathogen in humans. It lowers stomach acid levels while damaging the mucosal protection within the stomach. It has therefore been attributed with causing stomach and duodenal ulcers. 

Pancreatic elastase 1

Elastase findings can be used for the diagnosis or the exclusion of exocrine pancreatic insuffiency. Reduced pancreatic elastase in stool indicates insufficiency of exocrine pancreatic function. Pancreatic elastase 1 levels below 100 are strongly correlated with severe pancreatic insufficiency. Pancreatic elastase 1 levels between 100 and 200 indicate moderate pancreatic insufficiency.

Alpha-1-Antitrypsin

Alpha-1-antitrypsin is a glycoprotein, which is produced by the liver and cells of the gut. It belongs to the group of acute phase proteins and is a marker of of protein loss and permeability of the gut.The measurement of Alpha-1-antitrypsin in stool reflects the permeability of the gut during inflammatory processes. Increased Alpha-1-antitrypsin indicates an increased permeability of the intestinal mucous membrane, which leads to an enteral loss of Alpha-1-antitrypsin. 

Secretory IgA

Secretory IgA (sIgA) is an immune protein, which reacts anti-inflammatory. It coats the intestinal lining, especially the mucosal surfaces and is supposed to protect us from inside. As secretory IgA represents the first line of defense of the GI, immunological activity in the GI tract can be assessed using secretory IgA. Low levels of fecal sIgA increase the risk of leaky gut syndrome and promote the growth of microbial pathogens in the intestine. The risk of inflammatroy immune reactions to undigested food and protein is also increased if low levels of sIgA are present. Low fecal IgA levels can result from physical or mental stress and/or inadequate nutrition. High fecal sIgA indicate immune reactions to antigens from bacteria, yeast or other microbes.

• - Depressive moods

• - Fatigue or feeling tired

• - Constipation/ Swollen stomach

• - Diarrhea/ Constipation

• - Food intolerance

• - Burning stomach acid

• - Sensitive areas on the body 

• - Fungal infections on the skin or nails/ Eczema

• - Physical exhaustion

• - Burning sensation when urinating

• - Pain during intercourse

• - Vaginal itching

• - Menstrual disorders

• - Prostate problems 

• - Bladder infections

• - Hemorrhoids

• - Chronically stuffy nose

• - Bad breath

• - Sleeping disorders

• - Chronic diseases

• - Joint pain

• - Muscle pain

• - Increased suspectibility for infection

• - Uncertainty

• - Nervousness/ Anxiety

• - Forgetfulness

Please make sure to only dispatch your samples to our laboratory on Mondays!

Female Hormone Health Check

Before the saliva collection

General

-Only conduct the saliva test on a day which matches the time schemes for the sample collection.
-We strongly advise patients with gum diseases and gingivitis against using a saliva test.
-Always make sure to thoroughly wash your hands when using hormone creams. Due to the sensitivity of the saliva test we advise wearing disposable gloves for the collection of the samples.
-Please pay attention to the following time requirements referring to the examination of the hormones DHEA, progesterone, estradiol, estriol and testosterone.

Collecting time for women:

-With regular menstruation: On the 19th - 21st day of the cycle (calculated from the first day of the menstruation)

-With irregular menstruation: 5 days before the expected menstruation

-Without menstruation: Any day of the month

-With contraceptives that determine a 21 day cycle with a 7 day pause. In the first two weeks (calculated from the first day of taking the pill)

-With contraceptives determine a long-term cycle: Any day of the month

Collecting time for men: Any day of the month

Two to five days before the collection

-From now on please swallow all sublingual hormones such as drops, pastilles and so on directly – and do not allow to remain in the mouth under any circumstances.
-Please stop the use of any hormonol gels or creams 48 hours before the sample collection.

12 to 24 hours before the collection

-Please refrain from the intake of all hormones, in the form of tablets and capsules, 12 hours before the collection of the samples (exception: contraceptive pill).
-No dental measures should be undertaken up to 24 hours beforehand.
-Please do not consume any alcohol as well as sour and very sugary food products from now on.
-The last consumption of animal products should be at least 12 hours before the saliva collection.

 On the day of the collection:

-Please do not brush your teeth before collecting the saliva.

-Do not eat any chocolate, onions, garlic, or cruciferous vegetables such as cauliflower, broccoli, cabbage, as well as milk or egg products.

-If you are collecting the saliva over the day then please adopt to a vegetarian diet.

-Do not drink coffee, tea or other caffeinated beverages.

-Please do not smoke.

During the last 60 minutes before the collection:

-Please do not consume any more meals or liquids (apart from water); please do not consume any more milk products.

-Sublingual hormones are only to be used after a successful collection of the saliva.

-10 minutes before the saliva collection the mouth should be throughly rinsed with water and be completely empty at least 5 minutes before the collection. This step may be ignored when collecting the sample at 2 am or when collecting directly after getting up.

-The mouth should not contain any leftovers from food or drink.

Daytime scheme for obtaining samples

All samples are to be collected on the same day!

Saliva sample 1: Directly after waking up
Saliva sample 2: 30 minutes after waking up
Saliva sample 3: 1 hour after waking up

During the saliva collection

1. Please label all the sample containers as well as the enclosed sample dispatch note with your first and last name, the date as well as the time of the saliva sample collection.

2. Please take one straw and the sample container out of the packaging. Put the straw in your mouth and use it to transport the saliva into the sample container.

3. Fill the sample container at least half way (preferably ¾), not including the foam. If you have collected too much foam then lightly tap against the sample container so that the foam can separate itself from the saliva liquid.

4. Check whether the sample has taken on a red colouring. If so, then this most likely is blood (for example due to bleeding gums), which would falsify the results. In this case, please dispose the sample, rinse the sample container with tap water and collect another sample (colourless) after 5 – 10 minutes.

5. Now close the lid of the sample container. Dispose the saliva tube in your household trash.

6. Place the sample in the delivered protective bag (clear bag with blue border). Then place everything together with the enclosed sample dispatch note in the grey plastic envelope and seal it.

You can leave the saliva sample stored in the refrigerator until dispatch. 

Comprehensive Gut Biome & Health Test

General

Please note the following guidelines regarding the stool collection - the correct collection of the stool is important for achieving analtered laboratory results! Please make sure to collect the samples on Mondays and post them the same day. 

Mouth swab

In addition to the intestinal flora analysis for analysing yeasts and moulds, we are also willing to examine your mouth swab free of charge. Please send the allocated tube together with the stool sample. Separate deliveries will not be taken into consideration.

The mouth should not be treated with an antifungal or antibacterial solution before the mouth swab. Remove the cap at the upper end of the tube along the dashed line. Move the cotton end of the stick around on your tongue a couple of times and insert it into the tube.

Before the sample collection 

1. Label the sample container with your full name, the date as well as the time of the sample collection.

2. No additional sealing or stapling of the sample tubes and delivery bag is necessary.

During the sample collection 

1. Please use the enclosed stool collection unit to avoid the stool coming in contact with toilet water or urine. Now collect the sample using the stool collection unit.

2. Take the enclosed collection spoon (attached underneath the lid) in order to stir the stool. Collect different samples from different areas of the stool.

3. Fill the container with the stool sample until the tube is ¾ full.

4. Close the sample container tightly with the lid and clean the outside surface if necessary

After the sample collection 

1. After the collection, the stool collection unit as well as the remaining stool can be flushed down the toilet. 

2. The sample can be placed in the refrigerator over night – please do not freeze!

3. Please collect the sample on Mondays and post them the same day.

4. Place the sample in the delivered protective bag (clear bag with blue border). Place the sample together with the enclosed sample dispatch note in the delivered protective bag and send it back to our laboratory. In accordance with the general postal regulations the sender bears the legal responsibility.

5. After approximately 14-21 days you will receive your results.

Questions about the order

• Which test should I order?
• Can I also order by phone?
• How long does it take for my order to arrive?
• I have not received an order confirmation.
• My package did not arrive or my order was not completely delivered.
• I want to cancel my order.

Questions about sample submission & findings

• How long will it take to receive my findings?
• How do I get my findings?
• Can I get advice for my test result? Can you tell me what remedy to take?
• I forgot to put the sample dispatch note in the package when sending the sample back.
• I take medication. Can this affect the outcome?

Questions about the saliva test

• My cycle is so irregular that I can not determine when being about one week before the menstruation.
• I would like to do the test on a Friday/weekend, but in the instructions it is written that you should send the samples only at the beginning of the week.
• How long can you store or use saliva samples?
• What is the difference between a hormone saliva test and a blood hormone test, and why can the results vary widely?

Questions about the stool test

• How long can you store or use stool samples?
• I take probiotika. Does this affect the outcome?

Which test should I order?

To find the right test for you, click through our additional product information covered in the categories <Details>, <Symptoms> and <Test includes>. You can also call us at +44 (0) 20 32866316 or write an email to contact@verisana.co.uk.

Can I also order by phone?

Yes. Please call us at +44 (0) 20 32866316. The payment in this case is only possible via Paypal. We take your order and you will receive the payment request by mail.

How long does it take for my order to arrive?

We process orders on a daily basis (apart from weekends and public holidays). It usually takes around 3-5 days until your testkit arrives.

I have not received an order confirmation.

Please contact us to check if your order was registered. We will gladly send you the confirmation again.

My package did not arrive or my order was not completely delivered.

Please contact our customer service team at +44 (0) 20 32866316 or write an email to contact@verisana.co.uk.

I want to cancel my order. 

The withdrawal period from the purchase of all our items is 2 weeks from the receipt of the goods. Please inform us via mail about the desired cancellation and note the following information:  Laboratory analyzes can only be canceled if the analysis has not yet been carried out. 

How long will it take to receive my findings?

From the day the sample arrives in our laboratory, it takes:

- saliva tests 2 weeks 
- stool tests 2-3 weeks

until the findings are completed.

If you have not received your report after this deadline, please contact us. Please understand that we can not speed up the processing of your sample.

How do I get my findings?

You will receive your findings via mail.

Can I get advice for my test result? Can you tell me what remedy to take?

For legal reasons, our findings do not include individualized therapy recommendations. 

If you have any questions regarding the understanding of your findings, please contact our laboratory team.

I forgot to put the sample dispatch note in the package when sending the sample back.

Please send us the completed and signed dispatch note, preferably by mail to contact@verisana.co.uk. Unfortunately, we can not complete your sample without a dispatch note.

I take medication. Can this affect the outcome?

That depends on the test you want to carry out and the medications you are taking. In case of doubt, please ask your doctor regarding the areas that are influenced by the medication prescribed by him and do not stop taking your medication without consulting him.

My cycle is so irregular that I can not determine when being about one week before the menstruation.

If you can estimate when you are going to be in the second cycle phase, the test should be carried out in this second cycle phase.

If that also can not be assessed, samples can be taken on any day. However, in this case we can not classify whether progesterone and estradiol are in the normal range as this is dependent on the cycle phase.

I would like to do the test on a Friday/weekend,

but in the instructions it is written that you should send the samples only at the beginning of the week. 

It is best to store the samples in the fridge or freezer and send them out next Monday. So they do not stay at the post office over the weekend.

How long can you store or use saliva samples?

In principle, it is recommended to send the samples back as soon as possible after collection. In the refrigerator the samples last several weeks, frozen they even last months. Samples that are being transported for a longer time can still be used up to two weeks after collection.

What is the difference between a hormone saliva test and a blood hormone test, and why can the results vary widely?

Hormone saliva tests and blood hormone tests are two different test types. In contrast to saliva tests, blood tests are unable to differentiate the protein-bound (inactive) from the freely available and active hormones and thus give an approximate value.Therefore, the results of saliva tests and blood tests are not comparable with each other.

Measuring bio-available, free hormones

In comparison to the measurement of the bound, biological, inactive steroid hormones in blood, the determination of the steroid hormones using a salivary hormone test has the following essential advantages. When using saliva for the determination of the hormones, the free bio-available proportion of the hormones is measured. This is the part of the hormones that is not attached to proteins and which can be actively used by our cells, for example in the brain, the uterus or skin. 

Easy sample collection without supervision

The collection of the saliva sample is neither restricted by a certain place, nor by the consultation hours of your doctor, meaning you can collect the samples everywhere (at home, at work, under way). Hormones are periodically released in bigger amounts over the course of the day and are subject to daytime fluctuations, making it sensible to take mixed samples at different times during the day to obtain an average value.

In comparison to blood tests the saliva samples have a high stability because the accuracy of the results is not manipulated by being stored over several days (about a week) or by room temperature. In the refrigerator (35-46°F) or the freezer (-4°F) the durability of the samples is extended by weeks (refrigerator) or months (freezer). Blood, on the other hand, must be cooled and sent to the laboratory as soon as possible. To avoid a reproduction of the bacteria, the saliva samples should be stored in the refrigerator or freezer until dispatch. A continuous freezing and thawing of the samples has no effect.

Pain-free experience

Due to a non-invasive sample collection, patients experience neither pain nor stress, in contrast to a blood test, where the puncturing of a vein is necessary.
Consequently, the negative associations, such as the fear of needles or seeing blood, and pain or bruises (hematomas), involved with the puncture of the needle are completely eliminated.

This particularly is an advantage when dealing with children, young or frightened patients. Due to the non-invasive sample collection (no puncturing with needles necessary!) no medical assistance is needed.

Flexbility & Round-the-clock collection

Due to the temporal and spatial independence of the sample collection, the saliva sample can be collected at times that may have posed problems with the collection of blood samples, for example several times a day or at night. The salivary hormone test eases the creation of daily, nightly or monthly profiles. In this case the samples can be collected several times a day (for example for the recording of the daily rhythmic fluctuations of cortisol), in the night (e.g. melatonin and cortisol at 2 am in the case of sleeping disorders) or daily over a month (e.g, estradiol and progesterone over the monthly cycle in the case of a pre-menstrual syndrome).

How long can you store or use stool samples?

Basically, it is recommended to send the samples as soon as possible after collection and keep them in the refrigerator until they are shipped (do not freeze). On the way, the samples will last up to 7 days without any problems, when it is not too hot outside.

I take probiotika. Does this affect the outcome?

Taking probiotika means supplying bacteria to your body. In conclusion, carrying out a stool test while taking probiotika will make it unpossible to distinguish the amount of natural and unnatural bacteria in your gut. If you aim to find out about the amount of natural bacteria in your gut, we recommend you to stop taking probiotika two weeks before carrying out the stool test. 

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